The hydrolysis of Avicel PH 101 was performed by endoglucanase 1, exoglucanase Ⅱand their mixture from Trichoderma viride in the presence of 20 mM metal ions such as Mg^++, Cd^++, Zn^++, Ba^++, Ca^++, Hg^++, Pb^++. The addition of Mg^++, Ba^++, and Ca^++ on hydrolysis of Cellulose caused to increase on the total reducing sugar (TRS). On the other hand, significant decrease of TRS on the hydrolysis was observed with Zn^++, Hg^++ and Pb^++. The Ba^++ was the most stimulating metal ion whereas Hg^++ was the most inhibiting metal ion. Endo I was mere sensitive to these metal ions than exe Ⅱ. The physical strength of recycling Pulp and intact paper of old book treated by cellulase components(endo 1, exo Ⅱ and their mixture) was measured in the presence of inhibitory metal ion(Zn^++, Hg^++, Pb^++). And the physical strength was not decreased in the presence of Hg^++, Pb^++. From the experimental results, it was found that metal ions such as HB^++, Pb^++ were more efficient to prevent damage of paper cultural properties by cellulase.

Avicel PH 101의 가수분해가 20 mM의 Mg^++, Cd^++, Zn^++, Ba^++, Ca^++, Hg^++, Pb^++와 같은 금속이온 존재 하에 Trichoderma viride에서 분리한 endoglucanase I, exoglucanase Ⅱ와 endo-exo 혼합요소(1 : 1, weight ratio)에 의하여 수행되었다. 가수분해 시 Mg^++, Ba^++와 Ca^++첨가는 상대적인 총환원당(relative TRS)을 증가시켰으며, Zn^++, Hg^++과 Pb^++의 첨가는 총 환원당을 크게 감소시켰다. Ba^++는 최대 활성물질로, Hg^++는 최대활성 저해물질로 나타났다. Endo I은 exo Ⅱ보다 이들 금속이온의 영향을 더 크게 받는 것으로 나타났다. 활성 저해 금속이온인 Zn^++, Hg^++과 Pb^++의 존재 하에 셀롤라아제로 처리한 고서적의 재생펄프와 종이(intact paper)의 물리적 강도가 측정되었으며, Hg^++과 Pb^++이 존재 할 경우 물리적 강도의 저하를 초래하지 않았다. 이들 결과로부터 Hg^++과 Pb^++는 지류문화재 보존에 있어서 셀롤라아제에 의한 손상을 막을 수 있는 효과적인 물질로 나타났다.