In this study, the reduction of indigo to leuco-indigo by glucose was significantly enhanced using CdSe/ZnS core-shell quantum dots (QDs) and the polymerized mixture of two silanes, 3-glycidoxypropyl trimethoxysilane (GPTMS) and 3-aminopropyltrimeth- oxysilane (APTMS), namely GA. The efficiency of the indigo reduction to leuco-indigo was significantly higher in the presence of QDs, GA, and UV irradiation than with glucose only. The working mechanism of QDs and GA as catalysts in the indigo reduction by glucose is attributed to the reaction of electrons produced from the electron-hole pair recombination of the excited QDs under UV irradiation and the degradation of sol-gel GA in an alkaline medium to indigo molecules, which improved the electron flow between glucose and indigo. The concept of using inexpensive and non-toxic materials as catalysts to increase the efficiency of glucose in indigo reduction may be developed soon.