This study focused on optimizing the manufacturing conditions of cosmeceuticals with emulsion stability and antimicrobial properties by using the extract of fir and pine as natural preservatives. The optimization process was performed using the central composite design-response surface methodology (CCD-RSM). The CCD-RSM analysis yielded a highly reliable regression equation. The optimization results showed that the optimal manufacturing conditions for cosmeceuticals were an emulsifier HLB value of 9.3 and a fir extract ratio of 0.47. Under these conditions, the predicted response values were an ESI of 94.3% and inhibition diameters against E. coli and S. aureus of 8.0 and 8.9 mm, respectively. When cosmeceuticals were manufactured under the optimized conditions and the response values were measured, the average error rate was found to be within ± 0.96%. Thus, by applying the CCD-RSM optimization process, optimal manufacturing conditions for cosmeceuticals with excellent stability and antimicrobial properties were successfully achieved.