4-t-butyl phenol (BP), 4-n-pentyl phenol (PP), 4-n-hexyl phenol (HxP), 4-1-octyl phenol (t-OP), 4-n-heptyl phenol (HpP), b-nonyl phenol (b-NP), 4-n-octyl phenol (OP), and 4-n-nonyl phenol (NP) were simultaneously separated by a high performance liquid chromatography (HPLC) with UV diode array detector(230 nm, f60 nm, 280 nm) using an octadecylsilica column (Cl8, 250×4.6 mm) and water/methanol gradient solution. The calibration curves of alkyl phenols showed a good linear relationship, and the recoveries were over 95%. With the detection limit of 1pg for alkyl phenols with respect to the injected amount, alkyl phenols were not detected in tile dishwashing detergents that were available on the market.

본 연구는 알킬 페놀류 8종(4-t-butyl phenol (BP), 4-n-pentyl phenol (PP), 4-n-hexyl phenol (HxP), 4-t-octyl phenol (t-OP), 4-n-heptyl phenol (HpP), b-nonyl phenol (b-NP). 4-n-octyl phenol (OP), 4-n-nonyl phenol (NP))을 액체 크로마토그래피(HPLC) 다이오드 배열 검출기(diode array detector, DAD)를 이용하여 동시분석하였다. 분리관은 역상 Cl8(250×4.6 mm), 이동상은 물과 메탄올을 사용하여 기울기 용리(gradient)하에서 알킬 페놀류의 알킬 길이에 따라 분리하였으며, 알킬 페놀류의 검량선을 도시한 결과 좋은 직선성이 보였다. 상기 분석법으로 국내 유통 중인 주방 세제류 내 알킬 패놀류를 분석한 결과 불검출 되었고, 이때 검출한계는 주입량(10 μL) 대비 1 pg이었다.